10.1021/ja0491836.s001 J. David Warren J. David Warren Justin S. Miller Justin S. Miller Stacy J. Keding Stacy J. Keding Samuel J. Danishefsky Samuel J. Danishefsky Toward Fully Synthetic Glycoproteins by Ultimately Convergent Routes:  A Solution to a Long-Standing Problem American Chemical Society 2004 bifunctional glycopeptide convergent technique acyl donor approach centers Synthetic Glycoproteins convergent segment acid residue glycosylated peptides convergent synthesis 2004-06-02 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Toward_Fully_Synthetic_Glycoproteins_by_Ultimately_Convergent_Routes_A_Solution_to_a_Long_Standing_Problem/3337237 A method is disclosed for the convergent synthesis of multiply glycosylated peptides. The approach centers on a convergent technique for generating masked, complex glycopeptide-containing C-terminal acyl donors. Activation of the latent donor in situ and use directly in segment coupling with a second peptide bearing a complex carbohydrate produces a completely unprotected, bifunctional glycopeptide. The system demonstrates a minimum level of hydrolysis and epimerization at the C-terminal amino acid residue of the acyl donor during fully convergent segment coupling and is therefore a powerful tool for the synthesis of glycoproteins.