Global Analysis of the Membrane Subproteome of
<i>Pseudomonas aeruginosa</i> Using Liquid Chromatography-Tandem Mass
Spectrometry
Josip Blonder
Michael B. Goshe
Wenzhong Xiao
David G. Camp
Mark Wingerd
Ronald W. Davis
Richard D. Smith
10.1021/pr034074w.s002
https://acs.figshare.com/articles/journal_contribution/Global_Analysis_of_the_Membrane_Subproteome_of_i_Pseudomonas_aeruginosa_i_Using_Liquid_Chromatography_Tandem_Mass_Spectrometry/3335863
<i>Pseudomonas aeruginosa</i> is one of the most significant opportunistic bacterial pathogens in humans
causing infections and premature death in patients with cystic fibrosis, AIDS, severe burns, organ
transplants, or cancer. Liquid chromatography coupled online with tandem mass spectrometry was
used for the large-scale proteomic analysis of the <i>P. </i><i>aeruginosa</i> membrane subproteome. Concomitantly,
an affinity labeling technique, using iodoacetyl-PEO biotin to tag cysteinyl-containing proteins, permitted
the enrichment and detection of lower abundance membrane proteins. The application of these
approaches resulted in the identification of 786 proteins. A total of 333 proteins (42%) had a minimum
of one transmembrane domain (ranging from 1 to14) and 195 proteins were classified as hydrophobic
based on their positive GRAVY values (ranging from 0.01 to 1.32). Key integral inner and outer
membrane proteins involved in adaptation and antibiotic resistance were conclusively identified,
including the detection of 53% of all predicted opr-type porins (outer integral membrane proteins) and
all the components of the mexA-mexB-oprM transmembrane protein complex. This work represents
one of the most comprehensive proteomic analyses of the membrane subproteome of <i>P. </i><i>aeruginosa</i>
and for prokaryotes in general.
Keywords: proteome • membrane proteins • low abundance • LC−MS/MS • affinity labeling
2004-06-14 00:00:00
integral membrane proteins
195 proteins
tandem mass spectrometry
Membrane Subproteome
GRAVY values
786 proteins
proteomic analysis
abundance membrane proteins
membrane proteins
transmembrane domain
aeruginosa membrane subproteome
Liquid chromatography
AIDS
membrane subproteome
LC
proteomic analyses
organ transplants
Pseudomonas aeruginosa
antibiotic resistance
Global Analysis