Ruthenium(II)−Phenanthroline−Biotin Complexes: Synthesis and
Luminescence Enhancement upon Binding to Avidin
Mohamed Slim
Hanadi F. Sleiman
10.1021/bc049919o.s001
https://acs.figshare.com/articles/journal_contribution/Ruthenium_II_Phenanthroline_Biotin_Complexes_Synthesis_and_Luminescence_Enhancement_upon_Binding_to_Avidin/3325147
We report the synthesis, characterization, and avidin-binding properties of two novel ruthenium
complexes, [Ru(bpy)<sub>2</sub>(phen-biotin)][PF<sub>6</sub>]<sub>2</sub> <b>1</b> and [Ru(phen)<sub>2</sub>(phen-biotin)][PF<sub>6</sub>]<sub>2</sub> <b>2</b> (bpy = 2,2‘-bipyridine;
phen = 1,10-phenanthroline, phen-biotin = 5-(10-amidobiotinyl)-1,10-phenanthroline)). We demonstrate that both biotinylated compounds bind to avidin through their biotin moieties with high affinity
and in a 4:1 ratio. The binding of compounds <b>1</b> and <b>2</b> to avidin results in an enhancement in
luminescence intensity (∼1.4×, ∼1.6×, respectively), relative to the unbound biotinylated ruthenium
complexes. This behavior is markedly different from biotinylated organic dyes, whose fluorescence is
quenched upon binding to avidin. Thus, ruthenium−biotin complexes <b>1</b> and <b>2</b> can form the basis of
new, simplified biotin−avidin assays, which involve luminescence detection of the relevant biotinylated
molecule through cross-linking with avidin.
2004-09-15 00:00:00
Ru
biotin moieties
biotinylated compounds bind
2 1
biotinylated ruthenium complexes
biotinylated molecule
luminescence detection
compounds 1
binding
avidin results
novel ruthenium complexes
Luminescence Enhancement
2 2