10.1021/bc049919o.s001 Mohamed Slim Mohamed Slim Hanadi F. Sleiman Hanadi F. Sleiman Ruthenium(II)−Phenanthroline−Biotin Complexes:  Synthesis and Luminescence Enhancement upon Binding to Avidin American Chemical Society 2004 Ru biotin moieties biotinylated compounds bind 2 1 biotinylated ruthenium complexes biotinylated molecule luminescence detection compounds 1 binding avidin results novel ruthenium complexes Luminescence Enhancement 2 2 2004-09-15 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Ruthenium_II_Phenanthroline_Biotin_Complexes_Synthesis_and_Luminescence_Enhancement_upon_Binding_to_Avidin/3325147 We report the synthesis, characterization, and avidin-binding properties of two novel ruthenium complexes, [Ru(bpy)<sub>2</sub>(phen-biotin)][PF<sub>6</sub>]<sub>2</sub> <b>1</b> and [Ru(phen)<sub>2</sub>(phen-biotin)][PF<sub>6</sub>]<sub>2</sub> <b>2</b> (bpy = 2,2‘-bipyridine; phen = 1,10-phenanthroline, phen-biotin = 5-(10-amidobiotinyl)-1,10-phenanthroline)). We demonstrate that both biotinylated compounds bind to avidin through their biotin moieties with high affinity and in a 4:1 ratio. The binding of compounds <b>1</b> and <b>2</b> to avidin results in an enhancement in luminescence intensity (∼1.4×, ∼1.6×, respectively), relative to the unbound biotinylated ruthenium complexes. This behavior is markedly different from biotinylated organic dyes, whose fluorescence is quenched upon binding to avidin. Thus, ruthenium−biotin complexes <b>1</b> and <b>2</b> can form the basis of new, simplified biotin−avidin assays, which involve luminescence detection of the relevant biotinylated molecule through cross-linking with avidin.