%0 Journal Article %A Shreder, Kevin R. %A Wong, Melissa S. %A Nomanbhoy, Tyzoon %A S. Leventhal, Phillip %A Fuller, Stacy R. %D 2004 %T Synthesis of AX7593, a Quinazoline-Derived Photoaffinity Probe for EGFR %U https://acs.figshare.com/articles/journal_contribution/Synthesis_of_AX7593_a_Quinazoline_Derived_Photoaffinity_Probe_for_EGFR/3320950 %R 10.1021/ol048656a.s001 %2 https://acs.figshare.com/ndownloader/files/5159740 %K 280 nM %K K b %K tetraethyleneglycol linker %K probe AX 7593. Photoaffinity %K EGFR inhibitors PP 2 %K photoaffinity probe %K AG %K AX 7593 %X The synthesis of a photoaffinity probe for EGFR is described. O-Alkylation of 4-(meta-azidoanilino)-6-methoxy-7-hydroxy-quinazoline with a protected tetraethyleneglycol linker followed by the attachment of tetramethylrhodamine yielded the fluorescent probe AX7593. Photoaffinity labeling of EGFR by AX7593 (Kb = 280 nM) was shown to have an efficiency of 34% and to be competitive with the EGFR inhibitors PP2 and AG1478. %I ACS Publications