Synthesis of AX7593, a Quinazoline-Derived Photoaffinity Probe for EGFR Kevin R. Shreder Melissa S. Wong Tyzoon Nomanbhoy Phillip S. Leventhal Stacy R. Fuller 10.1021/ol048656a.s001 https://acs.figshare.com/articles/journal_contribution/Synthesis_of_AX7593_a_Quinazoline_Derived_Photoaffinity_Probe_for_EGFR/3320950 The synthesis of a photoaffinity probe for EGFR is described. O-Alkylation of 4-(<i>meta</i>-azidoanilino)-6-methoxy-7-hydroxy-quinazoline with a protected tetraethyleneglycol linker followed by the attachment of tetramethylrhodamine yielded the fluorescent probe AX7593. Photoaffinity labeling of EGFR by <b>AX7593</b> (<i>K</i><sub>b</sub> = 280 nM) was shown to have an efficiency of 34% and to be competitive with the EGFR inhibitors PP2 and AG1478. 2004-10-14 00:00:00 280 nM K b tetraethyleneglycol linker probe AX 7593. Photoaffinity EGFR inhibitors PP 2 photoaffinity probe AG AX 7593