10.1021/ol048656a.s001 Kevin R. Shreder Kevin R. Shreder Melissa S. Wong Melissa S. Wong Tyzoon Nomanbhoy Tyzoon Nomanbhoy Phillip S. Leventhal Phillip S. Leventhal Stacy R. Fuller Stacy R. Fuller Synthesis of AX7593, a Quinazoline-Derived Photoaffinity Probe for EGFR American Chemical Society 2004 280 nM K b tetraethyleneglycol linker probe AX 7593. Photoaffinity EGFR inhibitors PP 2 photoaffinity probe AG AX 7593 2004-10-14 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Synthesis_of_AX7593_a_Quinazoline_Derived_Photoaffinity_Probe_for_EGFR/3320950 The synthesis of a photoaffinity probe for EGFR is described. O-Alkylation of 4-(<i>meta</i>-azidoanilino)-6-methoxy-7-hydroxy-quinazoline with a protected tetraethyleneglycol linker followed by the attachment of tetramethylrhodamine yielded the fluorescent probe AX7593. Photoaffinity labeling of EGFR by <b>AX7593</b> (<i>K</i><sub>b</sub> = 280 nM) was shown to have an efficiency of 34% and to be competitive with the EGFR inhibitors PP2 and AG1478.