%0 Journal Article
%A Wang, Jun
%A Jiang, Yaxin
%A Zhou, Cuisong
%A Fang, Xiaohong
%D 2005
%T Aptamer-Based ATP Assay Using a Luminescent
Light Switching Complex
%U https://acs.figshare.com/articles/journal_contribution/Aptamer_Based_ATP_Assay_Using_a_Luminescent_Light_Switching_Complex/3283588
%R 10.1021/ac050165w.s004
%2 https://acs.figshare.com/ndownloader/files/5121331
%K acid aptamers
%K protein detection
%K Ru
%K luminescence change
%K ATP detection
%K model target
%K ATP binding
%K recognition probes
%K method
%K DNA
%K biosensor development
%K Luminescent Light Switching Complex
%K multistep analysis
%K 1 nM
%K dppz
%X With the increasing applications of nucleic acid aptamers
as a new class of molecular recognition probes in bioanalysis and biosensor development, the development of
general and simple signaling strategies to transduce
aptamer−target binding events to detectable signals is
demanding. We have developed a new signaling method
based on aptamers and a DNA molecular light switching
complex, [Ru(phen)2(dppz)]2+, for sensitive protein detection. In this work, we have demonstrated the applicability of this signaling mechanism to small-molecule
detection using ATP as a model target. Our results have
shown that upon ATP binding to the folded aptamer where
[Ru(phen)2(dppz)]2+ intercalated, the conformational
change or distortion of the aptamer is large enough to
cause a significant luminescence change of [Ru(phen)2(dppz)]2+. By monitoring the ATP-dependent luminescence intensity change, we have achieved ATP detection
with high selectivity and high sensitivity down to 1 nM in
homogeneous solution. The method is very simple without
the needs for covalently labeling aptamers or using costly
enzymes and multistep analysis as other reported fluorescence/luminescence assays for ATP. The successful
detection of ATP indicates that using the signaling aptamers with [Ru(phen)2(dppz)]2+ is expected to be a general
method for aptamer-based target detection.
%I ACS Publications