Aptamer-Based ATP Assay Using a Luminescent
Light Switching Complex
Jun Wang
Yaxin Jiang
Cuisong Zhou
Xiaohong Fang
10.1021/ac050165w.s004
https://acs.figshare.com/articles/journal_contribution/Aptamer_Based_ATP_Assay_Using_a_Luminescent_Light_Switching_Complex/3283588
With the increasing applications of nucleic acid aptamers
as a new class of molecular recognition probes in bioanalysis and biosensor development, the development of
general and simple signaling strategies to transduce
aptamer−target binding events to detectable signals is
demanding. We have developed a new signaling method
based on aptamers and a DNA molecular light switching
complex, [Ru(phen)<sub>2</sub>(dppz)]<sup>2+</sup>, for sensitive protein detection. In this work, we have demonstrated the applicability of this signaling mechanism to small-molecule
detection using ATP as a model target. Our results have
shown that upon ATP binding to the folded aptamer where
[Ru(phen)<sub>2</sub>(dppz)]<sup>2+</sup> intercalated, the conformational
change or distortion of the aptamer is large enough to
cause a significant luminescence change of [Ru(phen)<sub>2</sub>(dppz)]<sup>2+</sup>. By monitoring the ATP-dependent luminescence intensity change, we have achieved ATP detection
with high selectivity and high sensitivity down to 1 nM in
homogeneous solution. The method is very simple without
the needs for covalently labeling aptamers or using costly
enzymes and multistep analysis as other reported fluorescence/luminescence assays for ATP. The successful
detection of ATP indicates that using the signaling aptamers with [Ru(phen)<sub>2</sub>(dppz)]<sup>2+</sup> is expected to be a general
method for aptamer-based target detection.
2005-06-01 00:00:00
acid aptamers
protein detection
Ru
luminescence change
ATP detection
model target
ATP binding
recognition probes
method
DNA
biosensor development
Luminescent Light Switching Complex
multistep analysis
1 nM
dppz