10.1021/ac050165w.s004
Jun Wang
Jun
Wang
Yaxin Jiang
Yaxin
Jiang
Cuisong Zhou
Cuisong
Zhou
Xiaohong Fang
Xiaohong
Fang
Aptamer-Based ATP Assay Using a Luminescent
Light Switching Complex
American Chemical Society
2005
acid aptamers
protein detection
Ru
luminescence change
ATP detection
model target
ATP binding
recognition probes
method
DNA
biosensor development
Luminescent Light Switching Complex
multistep analysis
1 nM
dppz
2005-06-01 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Aptamer_Based_ATP_Assay_Using_a_Luminescent_Light_Switching_Complex/3283588
With the increasing applications of nucleic acid aptamers
as a new class of molecular recognition probes in bioanalysis and biosensor development, the development of
general and simple signaling strategies to transduce
aptamer−target binding events to detectable signals is
demanding. We have developed a new signaling method
based on aptamers and a DNA molecular light switching
complex, [Ru(phen)<sub>2</sub>(dppz)]<sup>2+</sup>, for sensitive protein detection. In this work, we have demonstrated the applicability of this signaling mechanism to small-molecule
detection using ATP as a model target. Our results have
shown that upon ATP binding to the folded aptamer where
[Ru(phen)<sub>2</sub>(dppz)]<sup>2+</sup> intercalated, the conformational
change or distortion of the aptamer is large enough to
cause a significant luminescence change of [Ru(phen)<sub>2</sub>(dppz)]<sup>2+</sup>. By monitoring the ATP-dependent luminescence intensity change, we have achieved ATP detection
with high selectivity and high sensitivity down to 1 nM in
homogeneous solution. The method is very simple without
the needs for covalently labeling aptamers or using costly
enzymes and multistep analysis as other reported fluorescence/luminescence assays for ATP. The successful
detection of ATP indicates that using the signaling aptamers with [Ru(phen)<sub>2</sub>(dppz)]<sup>2+</sup> is expected to be a general
method for aptamer-based target detection.