Detection of Fragmented Genomic DNA by PCR-Free Piezoelectric Sensing
Using a Denaturation Approach
Maria Minunni
Sara Tombelli
Jessica Fonti
Maria M. Spiriti
Marco Mascini
Patrizia Bogani
Marcello Buiatti
10.1021/ja051345q.s001
https://acs.figshare.com/articles/journal_contribution/Detection_of_Fragmented_Genomic_DNA_by_PCR_Free_Piezoelectric_Sensing_Using_a_Denaturation_Approach/3283105
Label-free and real-time DNA sequence detection in PCR-amplified DNA samples can now be achieved by different approaches. On the contrary, only few works have been reported dealing with direct sequence detection in nonamplified genomic DNA. Here, a piezoelectric biosensor for direct detection of sequences in nonamplified genomic DNA is described. The system relies on real-time and label-free detection of the hybridization reaction between an immobilized probe and the complementary sequence in solution. The DNA probe is immobilized on the sensing surface (10 MHz quartz crystals), while the complementary sequence is present in the genomic DNA, previously fragmented with restriction enzymes.
2005-06-08 00:00:00
nonamplified genomic DNA
Fragmented Genomic DNA
restriction enzymes
DNA sequence detection
hybridization reaction
DNA probe
sequence detection
genomic DNA