Pseudotrienic Acids A and B, Two Bioactive Metabolites from <i>Pseudomonas</i> sp. MF381-IODS PohankaAnton BrobergAnders JohanssonMaria KenneLennart LevenforsJolanta 2005 Bioassay-guided fractionation of the liquid culture broth of <i>Pseudomonas</i> sp. MF381-IODS yielded two new antimicrobial substances, identified as (2<i>E</i>,4<i>E</i>,6<i>E</i>)-9-[((2<i>S</i>,3<i>R</i>)-3-hydroxy-4-{[(3<i>E</i>,5<i>E</i>,7<i>RS</i>)-7-hydroxy-4-methylhexadeca-3,5-dienoyl]amino}-2-methylbutanoyl)amino]nona-2,4,6-trienoic acid and the tetradeca equivalent, named pseudotrienic acids A (<b>1</b>) and B (<b>2</b>), respectively. The compounds are prone to lactone formation, and their structures suggest them to be derived from ring opening of a macrolide. Pseudotrienic acids A and B inhibited growth of <i>Staphylococcus aureus</i> (MIC 70 μg/mL) and <i>Pseudomonas</i> <i>syringae </i>pv<i>. syringae </i>(MIC 70 μg/mL). Two known antimicrobial compounds, the polyketide 2,3-deepoxy-2,3-didehydrorhizoxin (<b>3</b>) and the tryptophan-derived pyrrolnitrin (<b>4</b>), were also identified.