Species-Specific Isotope Dilution Analysis and
Isotope Pattern Deconvolution for Butyltin
Compounds Metabolism Investigations
Pablo Rodríguez-González
Andrés Rodríguez-Cea
J. Ignacio García Alonso
Alfredo Sanz-Medel
10.1021/ac051091r.s001
https://acs.figshare.com/articles/journal_contribution/Species_Specific_Isotope_Dilution_Analysis_and_Isotope_Pattern_Deconvolution_for_Butyltin_Compounds_Metabolism_Investigations/3253723
A methodology for the study of the absorption and
metabolism of butyltin compounds in laboratory animals
using isotopically enriched species was developed. The
method is based on the oral administration of <sup>119</sup>Sn-labeled monobutyltin (MBT), <sup>118</sup>Sn-labeled dibutyltin
(DBT), and <sup>117</sup>Sn-labeled tributyltin (TBT) to the animals
and the measurement of both the concentration and
isotopic composition of these compounds in the different
tissues by GC−ICPMS. The degradation of butyltin compounds during their metabolism was computed using
least-squares isotope pattern deconvolution, and their
concentration was measured by reverse isotope dilution
analysis using natural-abundance MBT, DBT, and TBT
standards. Male Wistar rats were used as models to
evaluate the proposed methodology. Preliminary toxicological results obtained with one rat indicate that TBT is
highly absorbed (64.4%), and it is found in all organs with
relatively high levels in stomach and intestines. The
apparent absorption of DBT was 27.3% and was mainly
found in liver, kidney, and intestines. However, a large
proportion of the found DBT is formed from the degradation of TBT (∼40% of the found DBT in liver is degraded
TBT). The apparent absorption of MBT was found to be
12.5%, and the originally administered MBT was mainly
recovered in the feces. However, MBT was clearly detected in liver, kidney, stomach, intestines, and urine as
degradation products of DBT and TBT. Although a significant variability from rat to rat is expected to be
obtained, the analytical variability provided by this methodology is small enough to yield meaningful biological
results. The results obtained demonstrate that the developed methodology is able to follow qualitatively, quantitatively, and simultaneously the specific metabolic pathways of different species of a given element.
2005-12-01 00:00:00
absorption
degradation
GC
butyltin compounds
DBT
TBT
Male Wistar rats
Isotope Pattern Deconvolution
isotope dilution analysis
Butyltin Compounds Metabolism Investigations
methodology
Preliminary toxicological results
MBT