Activity of <i>Desulfitobacterium</i> sp. Strain Viet1 Demonstrates Bioavailability of 2,4-Dichlorophenol Previously Sequestered by the Aquatic Plant <i>Lemna minor</i> Jacqueline M. Tront Benjamin K. Amos Frank E. Löffler F. Michael Saunders 10.1021/es0514545.s001 https://acs.figshare.com/articles/journal_contribution/Activity_of_i_Desulfitobacterium_i_sp_Strain_Viet1_Demonstrates_Bioavailability_of_2_4_Dichlorophenol_Previously_Sequestered_by_the_Aquatic_Plant_i_Lemna_minor_i_/3243955 Aquatic plants take up and sequester organic contaminants such as chlorophenols through incorporation in cell wall materials and storage in vacuoles. The ultimate fate of plant-sequestered chlorophenols, however, remains unclear. This research investigated 2,4-dichlorophenol (2,4-DCP) sequestration by the aquatic plant <i>Lemna minor</i> and evaluated contaminant release and bioavailability after plant death and cellular disruption. <sup>14</sup>C-labeled 2,4-DCP was used to establish that contaminant removed from the aqueous phase was retained internal to <i>L. minor</i>. An assay with <i>Desulfitobacterium</i> sp. strain Viet1 was used to assess the readily bioavailable fraction of plant-sequestered 2,4-DCP and plant metabolites of 2,4-DCP. In plant-free systems, strain Viet1 dechlorinated 2,4-DCP to stoichiometric amounts of 4-chlorophenol (4-CP) as a stable and quantifiable end product. Anaerobic microcosms containing inactivated <i>L. minor</i>, which had accumulated 3.8 μmol of 2,4-DCP equivalents/g of plant material (fresh weight) during a preceding aerobic exposure, were inoculated with strain Viet1. After 118 d of incubation with strain Viet1, 43.5% (±1.4%) of the contaminant was recovered as 4-CP, indicating a large portion of plant-sequestered 2,4-DCP was bioavailable for dechlorination by strain Viet1. In contrast, 4-CP formation was not observed in autoclaved microcosms, and only 26.1% (±1.0%) of plant-sequestered 2,4-DCP was recovered in the aqueous phase. These findings demonstrate contaminant cycling between plants and microorganisms, and emphasize that understanding the mechanisms and pathways of contaminant sequestration by plants is critical for predicting long-term contaminant fate. 2006-01-15 00:00:00 contaminant DCP 3.8 μ mol Aquatic Plant Lemna chlorophenol cell wall materials CP quantifiable end product Strain Viet 1 Demonstrates Bioavailability Desulfitobacterium sp strain Viet 1 strain Viet 1.