10.1021/acsami.6b02837.s001 Seyed R. Tabaei Seyed R. Tabaei Wei Beng Ng Wei Beng Ng Sang-Joon Cho Sang-Joon Cho Nam-Joon Cho Nam-Joon Cho Controlling the Formation of Phospholipid Monolayer, Bilayer, and Intact Vesicle Layer on Graphene American Chemical Society 2016 coat graphene surface lipid monolayer lipid membrane architectures Graphene Exciting progress interfacing lipid membranes quartz crystal microbalance lipid bilayer coatings vesicle fusion method Intact Vesicle Layer surface functionalization strategies SALB 2016-04-19 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Controlling_the_Formation_of_Phospholipid_Monolayer_Bilayer_and_Intact_Vesicle_Layer_on_Graphene/3203836 Exciting progress has been made in the use of graphene for bio- and chemical sensing applications. In this regard, interfacing lipid membranes with graphene provides a high-sealing interface that is resistant to nonspecific protein adsorption and suitable for measuring biomembrane-associated interactions. However, a controllable method to form well-defined lipid bilayer coatings remains elusive, and there are varying results in the literature. Herein, we demonstrate how design strategies based on molecular self-assembly and surface chemistry can be employed to coat graphene surface with different classes of lipid membrane architectures. We characterize the self-assembly of lipid membranes on CVD-graphene using quartz crystal microbalance with dissipation, field-effect transistor, and Raman spectroscopy. By employing the solvent-assisted lipid bilayer (SALB) method, a lipid monolayer and bilayer were formed on pristine and oxygen-plasma-treated CVD-graphene, respectively. On these surfaces, vesicle fusion method resulted in formation of a lipid monolayer and intact vesicle layer, respectively. Collectively, these findings provide the basis for improved surface functionalization strategies on graphene toward bioelectronic applications.