10.1021/acsami.6b02837.s001
Seyed R. Tabaei
Seyed R.
Tabaei
Wei Beng Ng
Wei Beng
Ng
Sang-Joon Cho
Sang-Joon
Cho
Nam-Joon Cho
Nam-Joon
Cho
Controlling
the Formation of Phospholipid Monolayer, Bilayer, and Intact Vesicle
Layer on Graphene
American Chemical Society
2016
coat graphene surface
lipid monolayer
lipid membrane architectures
Graphene Exciting progress
interfacing lipid membranes
quartz crystal microbalance
lipid bilayer coatings
vesicle fusion method
Intact Vesicle Layer
surface functionalization strategies
SALB
2016-04-19 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Controlling_the_Formation_of_Phospholipid_Monolayer_Bilayer_and_Intact_Vesicle_Layer_on_Graphene/3203836
Exciting
progress has been made in the use of graphene for bio- and chemical
sensing applications. In this regard, interfacing lipid membranes
with graphene provides a high-sealing interface that is resistant
to nonspecific protein adsorption and suitable for measuring biomembrane-associated
interactions. However, a controllable method to form well-defined
lipid bilayer coatings remains elusive, and there are varying results
in the literature. Herein, we demonstrate how design strategies based
on molecular self-assembly and surface chemistry can be employed to
coat graphene surface with different classes of lipid membrane architectures.
We characterize the self-assembly of lipid membranes on CVD-graphene
using quartz crystal microbalance with dissipation, field-effect transistor,
and Raman spectroscopy. By employing the solvent-assisted lipid bilayer
(SALB) method, a lipid monolayer and bilayer were formed on pristine
and oxygen-plasma-treated CVD-graphene, respectively. On these surfaces,
vesicle fusion method resulted in formation of a lipid monolayer and
intact vesicle layer, respectively. Collectively, these findings provide
the basis for improved surface functionalization strategies on graphene
toward bioelectronic applications.