10.1021/acs.nanolett.6b00502.s001
Hannelore Bové
Hannelore
Bové
Christian Steuwe
Christian
Steuwe
Eduard Fron
Eduard
Fron
Eli Slenders
Eli
Slenders
Jan D’Haen
Jan
D’Haen
Yasuhiko Fujita
Yasuhiko
Fujita
Hiroshi Uji-i
Hiroshi
Uji-i
Martin vandeVen
Martin
vandeVen
Maarten Roeffaers
Maarten
Roeffaers
Marcel Ameloot
Marcel
Ameloot
Biocompatible Label-Free Detection of Carbon Black
Particles by Femtosecond Pulsed Laser Microscopy
American Chemical Society
2016
WL emission
CB particles
illumination
Carbon Black Particles
fluid
detection
Femtosecond Pulsed Laser Microscopy
2016-04-22 11:50:08
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Biocompatible_Label_Free_Detection_of_Carbon_Black_Particles_by_Femtosecond_Pulsed_Laser_Microscopy/3189058
Although
adverse health effects of carbon black (CB) exposure are generally
accepted, a direct, label-free approach for detecting CB particles
in fluids and at the cellular level is still lacking. Here, we report
nonincandescence related white-light (WL) generation by dry and suspended
carbon black particles under illumination with femtosecond (fs) pulsed
near-infrared light as a powerful tool for the detection of these
carbonaceous materials. This observation is done for four different
CB species with diameters ranging from 13 to 500 nm, suggesting this
WL emission under fs near-infrared illumination is a general property
of CB particles. As the emitted radiation spreads over the whole visible
spectrum, detection is straightforward and flexible. The unique property
of the described WL emission allows optical detection and unequivocal
localization of CB particles in fluids and in cellular environments
while simultaneously colocalizing different cellular components using
various specific fluorophores as shown here using human lung fibroblasts.
The experiments are performed on a typical multiphoton laser-scanning
microscopy platform, widely available in research laboratories.