%0 Journal Article
%A Cantley, Alexandra M.
%A Woznica, Arielle
%A Beemelmanns, Christine
%A King, Nicole
%A Clardy, Jon
%D 2016
%T Isolation and Synthesis of a Bacterially Produced
Inhibitor of Rosette Development in Choanoflagellates
%U https://acs.figshare.com/articles/journal_contribution/Isolation_and_Synthesis_of_a_Bacterially_Produced_Inhibitor_of_Rosette_Development_in_Choanoflagellates/3123592
%R 10.1021/jacs.6b01190.s001
%2 https://acs.figshare.com/ndownloader/files/4859110
%K chemical ecology
%K f r osettes
%K bacterially
%K multicellular states
%K stereoisomer
%K unicellular
%K specificity
%K laboratory
%K choanoflagellate Salpingoeca rosetta
%K i nhibitor
%K Rosette Development
%K Choanoflagellate
%K IOR
%K rosettes
%K identification
%K Bacterially Produced Inhibitor
%K Synthesi
%K multicellularity
%K Isolation
%K bacteria
%K molecule
%K understanding
%K probe template
%K sulfonolipid
%K emergence
%K marine eukaryote
%K potency
%K animal lineage
%K model
%K synthesis
%K organism
%X The choanoflagellate Salpingoeca rosetta is a microbial marine eukaryote
that can switch between unicellular and multicellular states. As one
of the closest living relatives of animals, this organism has become
a model for understanding how multicellularity evolved in the animal
lineage. Previously our laboratories isolated and synthesized a bacterially
produced sulfonolipid that induces S. rosetta to form multicellular “rosettes.” In this study, we
report the identification of a bacterially produced inhibitor of rosettes (IOR-1) as
well as the total synthesis of this molecule and all of its stereoisomers.
Our results confirm the previously noted specificity and potency of
rosette-modulating molecules, expand our understanding of the complex
chemical ecology between choanoflagellates and rosette-inducing bacteria,
and provide a synthetic probe template for conducting further mechanistic
studies on the emergence of multicellularity.
%I ACS Publications