%0 Figure %A Naves, Alliny F. %A Carmona-Ribeiro, Ana M. %A F. S. Petri, Denise %D 2007 %T Immobilized Horseradish Peroxidase as a Reusable Catalyst for Emulsion Polymerization %U https://acs.figshare.com/articles/figure/Immobilized_Horseradish_Peroxidase_as_a_Reusable_Catalyst_for_Emulsion_Polymerization/3027217 %R 10.1021/la061884o.s005 %2 https://acs.figshare.com/ndownloader/files/4729579 %K Si wafers %K ABTS %K ethylene glycol dimethacrylate %K Reusable Catalyst %K force microscopy %K emulsion polymerization %K AFM %K Emulsion PolymerizationThe study %K Immobilized Horseradish Peroxidase %K EGDMA %K HRP molecules %K horseradish peroxidase %K HRP layer %K HRP solution %K contact angle measurements %K silicon wafers %X The study on the adsorption of horseradish peroxidase (HRP) onto silicon wafers was carried out by means of in situ ellipsometry, atomic force microscopy (AFM) and contact angle measurements. A smooth HRP layer adsorbed onto Si wafers. The enzymatic activity of free or adsorbed HRP was determined by the oxidation of 2,2‘-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and by the emulsion polymerization of ethylene glycol dimethacrylate (EGDMA). Upon adsorbing, HRP molecules might have undergone some conformational changes, which caused a small reduction of enzymatic activity in comparison to that observed for HRP solution. However, it was possible to reuse the same HRP-covered Si wafer as catalyst in the polymerization of EGDMA three times. %I ACS Publications