%0 Figure
%A Naves, Alliny F.
%A Carmona-Ribeiro, Ana M.
%A F. S. Petri, Denise
%D 2007
%T Immobilized Horseradish Peroxidase as a Reusable Catalyst for
Emulsion Polymerization
%U https://acs.figshare.com/articles/figure/Immobilized_Horseradish_Peroxidase_as_a_Reusable_Catalyst_for_Emulsion_Polymerization/3027217
%R 10.1021/la061884o.s005
%2 https://acs.figshare.com/ndownloader/files/4729579
%K Si wafers
%K ABTS
%K ethylene glycol dimethacrylate
%K Reusable Catalyst
%K force microscopy
%K emulsion polymerization
%K AFM
%K Emulsion PolymerizationThe study
%K Immobilized Horseradish Peroxidase
%K EGDMA
%K HRP molecules
%K horseradish peroxidase
%K HRP layer
%K HRP solution
%K contact angle measurements
%K silicon wafers
%X The study on the adsorption of horseradish peroxidase (HRP) onto silicon wafers was carried out by means of in
situ ellipsometry, atomic force microscopy (AFM) and contact angle measurements. A smooth HRP layer adsorbed
onto Si wafers. The enzymatic activity of free or adsorbed HRP was determined by the oxidation of 2,2‘-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and by the emulsion polymerization of ethylene glycol dimethacrylate
(EGDMA). Upon adsorbing, HRP molecules might have undergone some conformational changes, which caused a
small reduction of enzymatic activity in comparison to that observed for HRP solution. However, it was possible to
reuse the same HRP-covered Si wafer as catalyst in the polymerization of EGDMA three times.
%I ACS Publications