Immobilized Horseradish Peroxidase as a Reusable Catalyst for
Emulsion Polymerization
Alliny F. Naves
Ana M. Carmona-Ribeiro
Denise F. S. Petri
10.1021/la061884o.s005
https://acs.figshare.com/articles/figure/Immobilized_Horseradish_Peroxidase_as_a_Reusable_Catalyst_for_Emulsion_Polymerization/3027217
The study on the adsorption of horseradish peroxidase (HRP) onto silicon wafers was carried out by means of <i>in
</i><i>situ</i> ellipsometry, atomic force microscopy (AFM) and contact angle measurements. A smooth HRP layer adsorbed
onto Si wafers. The enzymatic activity of free or adsorbed HRP was determined by the oxidation of 2,2‘-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and by the emulsion polymerization of ethylene glycol dimethacrylate
(EGDMA). Upon adsorbing, HRP molecules might have undergone some conformational changes, which caused a
small reduction of enzymatic activity in comparison to that observed for HRP solution. However, it was possible to
reuse the same HRP-covered Si wafer as catalyst in the polymerization of EGDMA three times.
2007-02-13 00:00:00
Si wafers
ABTS
ethylene glycol dimethacrylate
Reusable Catalyst
force microscopy
emulsion polymerization
AFM
Emulsion PolymerizationThe study
Immobilized Horseradish Peroxidase
EGDMA
HRP molecules
horseradish peroxidase
HRP layer
HRP solution
contact angle measurements
silicon wafers