Immobilized Horseradish Peroxidase as a Reusable Catalyst for Emulsion Polymerization Alliny F. Naves Ana M. Carmona-Ribeiro Denise F. S. Petri 10.1021/la061884o.s005 https://acs.figshare.com/articles/figure/Immobilized_Horseradish_Peroxidase_as_a_Reusable_Catalyst_for_Emulsion_Polymerization/3027217 The study on the adsorption of horseradish peroxidase (HRP) onto silicon wafers was carried out by means of <i>in </i><i>situ</i> ellipsometry, atomic force microscopy (AFM) and contact angle measurements. A smooth HRP layer adsorbed onto Si wafers. The enzymatic activity of free or adsorbed HRP was determined by the oxidation of 2,2‘-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and by the emulsion polymerization of ethylene glycol dimethacrylate (EGDMA). Upon adsorbing, HRP molecules might have undergone some conformational changes, which caused a small reduction of enzymatic activity in comparison to that observed for HRP solution. However, it was possible to reuse the same HRP-covered Si wafer as catalyst in the polymerization of EGDMA three times. 2007-02-13 00:00:00 Si wafers ABTS ethylene glycol dimethacrylate Reusable Catalyst force microscopy emulsion polymerization AFM Emulsion PolymerizationThe study Immobilized Horseradish Peroxidase EGDMA HRP molecules horseradish peroxidase HRP layer HRP solution contact angle measurements silicon wafers