10.1021/la063251f.s001
Carolyn M. McQuaw
Carolyn M.
McQuaw
Leiliang Zheng
Leiliang
Zheng
Andrew G. Ewing
Andrew G.
Ewing
Nicholas Winograd
Nicholas
Winograd
Localization of Sphingomyelin in Cholesterol Domains by Imaging
Mass Spectrometry
American Chemical Society
2007
sphingomyelin
phase
fluorescence microscopy literature
results show
label
phospholipid
Localization
Cholesterol Domains
laterally
coexisting immiscible
palmitoyloleoylphosphatidylcholine
lipid
ion mass spectrometry
Imaging Mass SpectrometryThe location
imaging
necessity
segregation
Sphingomyelin
cholesterol domains
SM localizes
monolayer
2007-05-08 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Localization_of_Sphingomyelin_in_Cholesterol_Domains_by_Imaging_Mass_Spectrometry/3009076
The location of each lipid in a palmitoyloleoylphosphatidylcholine/18:0 sphingomyelin/cholesterol monolayer
system is laterally resolved using imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS) without the
necessity of adding fluorescent labels. This system of coexisting immiscible liquid phases shows cholesterol domains
with sizes and shapes comparable to those in the fluorescence microscopy literature. The results show that SM localizes
with cholesterol and that palmitoyloleoylphosphatidylcholine is excluded. Moreover, the segregation is not complete,
and there is a small amount of both phospholipids distributed throughout.