Array-Based Sensing of Proteins Using Conjugated Polymers
Oscar R. Miranda
Chang-Cheng You
Ronnie Phillips
Ik-Bum Kim
Partha S. Ghosh
Uwe H. F. Bunz
Vincent M. Rotello
10.1021/ja0737927.s001
https://acs.figshare.com/articles/journal_contribution/Array_Based_Sensing_of_Proteins_Using_Conjugated_Polymers/2991490
A sensor array comprising six functionalized poly(<i>p</i>-phenyleneethynylene) polymers was constructed and used to detect and identify protein analytes. The presence of proteins alters the fluorescence properties of the polymers, generating a distinct fluorescence response pattern for a given protein. Linear discriminant analyses accurately differentiate the patterns of 17 proteins at nano<i>-</i> to micromolar concentrations. An identification accuracy of 97% was obtained on the basis of the tests with 68 unknown protein samples from the training set.
2007-08-15 00:00:00
polymer
protein analytes
17 proteins
fluorescence properties
identification accuracy
Conjugated PolymersA sensor array
fluorescence response pattern
protein samples
Linear discriminant analyses
micromolar concentrations