%0 Journal Article
%A Yin, Huabing
%A Pattrick, Nicola
%A Zhang, Xunli
%A Klauke, Norbert
%A Cordingley, Hayley C.
%A Haswell, Steven J.
%A Cooper, Jonathan M.
%D 2008
%T Quantitative Comparison between Microfluidic and
Microtiter Plate Formats for Cell-Based Assays
%U https://acs.figshare.com/articles/journal_contribution/Quantitative_Comparison_between_Microfluidic_and_Microtiter_Plate_Formats_for_Cell_Based_Assays/2965498
%R 10.1021/ac701958z.s002
%2 https://acs.figshare.com/ndownloader/files/4664635
%K screening protocols
%K hydrodynamic conditions
%K microtiter formats
%K subcelluar information
%K Quantitative Comparison
%K assay
%K Microtiter Plate Formats
%K Chinese hamster ovary cells
%X In this paper, we compare a quantitative cell-based assay
measuring the intracellular Ca2+ response to the agonist
uridine 5‘-triphosphate in Chinese hamster ovary cells,
in both microfluidic and microtiter formats. The study
demonstrates that, under appropriate hydrodynamic conditions, there is an excellent agreement between traditional well-plate assays and those obtained on-chip for
both suspended immobilized cells and cultured adherent
cells. We also demonstrate that the on-chip assay, using
adherent cells, provides the possibility of faster screening
protocols with the potential for resolving subcelluar
information about local Ca2+ flux.
%I ACS Publications