%0 Journal Article %A Yin, Huabing %A Pattrick, Nicola %A Zhang, Xunli %A Klauke, Norbert %A Cordingley, Hayley C. %A Haswell, Steven J. %A Cooper, Jonathan M. %D 2008 %T Quantitative Comparison between Microfluidic and Microtiter Plate Formats for Cell-Based Assays %U https://acs.figshare.com/articles/journal_contribution/Quantitative_Comparison_between_Microfluidic_and_Microtiter_Plate_Formats_for_Cell_Based_Assays/2965498 %R 10.1021/ac701958z.s002 %2 https://acs.figshare.com/ndownloader/files/4664635 %K screening protocols %K hydrodynamic conditions %K microtiter formats %K subcelluar information %K Quantitative Comparison %K assay %K Microtiter Plate Formats %K Chinese hamster ovary cells %X In this paper, we compare a quantitative cell-based assay measuring the intracellular Ca2+ response to the agonist uridine 5‘-triphosphate in Chinese hamster ovary cells, in both microfluidic and microtiter formats. The study demonstrates that, under appropriate hydrodynamic conditions, there is an excellent agreement between traditional well-plate assays and those obtained on-chip for both suspended immobilized cells and cultured adherent cells. We also demonstrate that the on-chip assay, using adherent cells, provides the possibility of faster screening protocols with the potential for resolving subcelluar information about local Ca2+ flux. %I ACS Publications