10.1021/ac701958z.s002 Huabing Yin Huabing Yin Nicola Pattrick Nicola Pattrick Xunli Zhang Xunli Zhang Norbert Klauke Norbert Klauke Hayley C. Cordingley Hayley C. Cordingley Steven J. Haswell Steven J. Haswell Jonathan M. Cooper Jonathan M. Cooper Quantitative Comparison between Microfluidic and Microtiter Plate Formats for Cell-Based Assays American Chemical Society 2008 screening protocols hydrodynamic conditions microtiter formats subcelluar information Quantitative Comparison assay Microtiter Plate Formats Chinese hamster ovary cells 2008-01-01 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Quantitative_Comparison_between_Microfluidic_and_Microtiter_Plate_Formats_for_Cell_Based_Assays/2965498 In this paper, we compare a quantitative cell-based assay measuring the intracellular Ca<sup>2+</sup> response to the agonist uridine 5‘-triphosphate in Chinese hamster ovary cells, in both microfluidic and microtiter formats. The study demonstrates that, under appropriate hydrodynamic conditions, there is an excellent agreement between traditional well-plate assays and those obtained on-chip for both suspended immobilized cells and cultured adherent cells. We also demonstrate that the on-chip assay, using adherent cells, provides the possibility of faster screening protocols with the potential for resolving subcelluar information about local Ca<sup>2+</sup> flux.