10.1021/ac701958z.s002
Huabing Yin
Huabing
Yin
Nicola Pattrick
Nicola
Pattrick
Xunli Zhang
Xunli
Zhang
Norbert Klauke
Norbert
Klauke
Hayley C. Cordingley
Hayley C.
Cordingley
Steven J. Haswell
Steven J.
Haswell
Jonathan M. Cooper
Jonathan M.
Cooper
Quantitative Comparison between Microfluidic and
Microtiter Plate Formats for Cell-Based Assays
American Chemical Society
2008
screening protocols
hydrodynamic conditions
microtiter formats
subcelluar information
Quantitative Comparison
assay
Microtiter Plate Formats
Chinese hamster ovary cells
2008-01-01 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Quantitative_Comparison_between_Microfluidic_and_Microtiter_Plate_Formats_for_Cell_Based_Assays/2965498
In this paper, we compare a quantitative cell-based assay
measuring the intracellular Ca<sup>2+</sup> response to the agonist
uridine 5‘-triphosphate in Chinese hamster ovary cells,
in both microfluidic and microtiter formats. The study
demonstrates that, under appropriate hydrodynamic conditions, there is an excellent agreement between traditional well-plate assays and those obtained on-chip for
both suspended immobilized cells and cultured adherent
cells. We also demonstrate that the on-chip assay, using
adherent cells, provides the possibility of faster screening
protocols with the potential for resolving subcelluar
information about local Ca<sup>2+</sup> flux.