A Simple Microscale Method for Determining the Relative Stereochemistry of Statine Units Alejandro Preciado Philip G. Williams 10.1021/jo8012429.s001 https://acs.figshare.com/articles/journal_contribution/A_Simple_Microscale_Method_for_Determining_the_Relative_Stereochemistry_of_Statine_Units/2894506 A simple method to determine the relative stereochemistry of statine amino acids (γ-amino-β-hydroxyacids) by using <sup>1</sup>H NMR spectroscopy is described. Configurational assignment of statine units within complex natural products is possible without degradation or derivatization as the <i>syn</i> and <i>anti</i> diastereomers can be distinguished by using a combination of chemical shift and coupling constant information derived from the α-methylene ABX system. Seventy-three examples are provided, demonstrating the scope and limitations of the methodology. These examples range in complexity from simple statine units to cyclic depsipeptides, such as tamandarin B. 2008-12-05 00:00:00 1 H NMR spectroscopy ABX Simple Microscale Method statine units