%0 Journal Article %A Iyer, Gopal %A Michalet, Xavier %A Chang, Yun-Pei %A Pinaud, Fabien F. %A Matyas, Stephanie E. %A Payne, Gregory %A Weiss, Shimon %D 2008 %T High Affinity scFv−Hapten Pair as a Tool for Quantum Dot Labeling and Tracking of Single Proteins in Live Cells %U https://acs.figshare.com/articles/journal_contribution/High_Affinity_scFv_Hapten_Pair_as_a_Tool_for_Quantum_Dot_Labeling_and_Tracking_of_Single_Proteins_in_Live_Cells/2893960 %R 10.1021/nl8032284.s001 %2 https://acs.figshare.com/ndownloader/files/4592101 %K scFv %K hapten fluorescein %K FL %K cell surface fusion proteins %K approach %K quantum dots %K Single Proteins %K Live CellsWe %K QD %K label cell surface proteins %K Quantum Dot %X We describe a general approach to label cell surface proteins using quantum dots (QD) for single-molecule tracking. QDs coated with small-hapten modified peptides are targeted to cell surface fusion proteins containing the corresponding single-chain fragment antibody (scFv). The approach is illustrated with the small hapten fluorescein (FL) and a high-affinity anti-FL scFv fused to two different proteins in yeast and murine neuronal cell line N2a. %I ACS Publications