High Affinity scFv−Hapten Pair as a Tool for Quantum Dot Labeling and Tracking of Single Proteins in Live Cells
Gopal Iyer
Xavier Michalet
Yun-Pei Chang
Fabien F. Pinaud
Stephanie E. Matyas
Gregory Payne
Shimon Weiss
10.1021/nl8032284.s001
https://acs.figshare.com/articles/journal_contribution/High_Affinity_scFv_Hapten_Pair_as_a_Tool_for_Quantum_Dot_Labeling_and_Tracking_of_Single_Proteins_in_Live_Cells/2893960
We describe a general approach to label cell surface proteins using quantum dots (QD) for single-molecule tracking. QDs coated with small-hapten modified peptides are targeted to cell surface fusion proteins containing the corresponding single-chain fragment antibody (scFv). The approach is illustrated with the small hapten fluorescein (FL) and a high-affinity anti-FL scFv fused to two different proteins in yeast and murine neuronal cell line N2a.
2008-12-10 00:00:00
scFv
hapten fluorescein
FL
cell surface fusion proteins
approach
quantum dots
Single Proteins
Live CellsWe
QD
label cell surface proteins
Quantum Dot