High Affinity scFv−Hapten Pair as a Tool for Quantum Dot Labeling and Tracking of Single Proteins in Live Cells Gopal Iyer Xavier Michalet Yun-Pei Chang Fabien F. Pinaud Stephanie E. Matyas Gregory Payne Shimon Weiss 10.1021/nl8032284.s001 https://acs.figshare.com/articles/journal_contribution/High_Affinity_scFv_Hapten_Pair_as_a_Tool_for_Quantum_Dot_Labeling_and_Tracking_of_Single_Proteins_in_Live_Cells/2893960 We describe a general approach to label cell surface proteins using quantum dots (QD) for single-molecule tracking. QDs coated with small-hapten modified peptides are targeted to cell surface fusion proteins containing the corresponding single-chain fragment antibody (scFv). The approach is illustrated with the small hapten fluorescein (FL) and a high-affinity anti-FL scFv fused to two different proteins in yeast and murine neuronal cell line N2a. 2008-12-10 00:00:00 scFv hapten fluorescein FL cell surface fusion proteins approach quantum dots Single Proteins Live CellsWe QD label cell surface proteins Quantum Dot