10.1021/nl8032284.s001 Gopal Iyer Gopal Iyer Xavier Michalet Xavier Michalet Yun-Pei Chang Yun-Pei Chang Fabien F. Pinaud Fabien F. Pinaud Stephanie E. Matyas Stephanie E. Matyas Gregory Payne Gregory Payne Shimon Weiss Shimon Weiss High Affinity scFv−Hapten Pair as a Tool for Quantum Dot Labeling and Tracking of Single Proteins in Live Cells American Chemical Society 2008 scFv hapten fluorescein FL cell surface fusion proteins approach quantum dots Single Proteins Live CellsWe QD label cell surface proteins Quantum Dot 2008-12-10 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/High_Affinity_scFv_Hapten_Pair_as_a_Tool_for_Quantum_Dot_Labeling_and_Tracking_of_Single_Proteins_in_Live_Cells/2893960 We describe a general approach to label cell surface proteins using quantum dots (QD) for single-molecule tracking. QDs coated with small-hapten modified peptides are targeted to cell surface fusion proteins containing the corresponding single-chain fragment antibody (scFv). The approach is illustrated with the small hapten fluorescein (FL) and a high-affinity anti-FL scFv fused to two different proteins in yeast and murine neuronal cell line N2a.