10.1021/nl8032284.s001
Gopal Iyer
Gopal
Iyer
Xavier Michalet
Xavier
Michalet
Yun-Pei Chang
Yun-Pei
Chang
Fabien F. Pinaud
Fabien F.
Pinaud
Stephanie E. Matyas
Stephanie E.
Matyas
Gregory Payne
Gregory
Payne
Shimon Weiss
Shimon
Weiss
High Affinity scFv−Hapten Pair as a Tool for Quantum Dot Labeling and Tracking of Single Proteins in Live Cells
American Chemical Society
2008
scFv
hapten fluorescein
FL
cell surface fusion proteins
approach
quantum dots
Single Proteins
Live CellsWe
QD
label cell surface proteins
Quantum Dot
2008-12-10 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/High_Affinity_scFv_Hapten_Pair_as_a_Tool_for_Quantum_Dot_Labeling_and_Tracking_of_Single_Proteins_in_Live_Cells/2893960
We describe a general approach to label cell surface proteins using quantum dots (QD) for single-molecule tracking. QDs coated with small-hapten modified peptides are targeted to cell surface fusion proteins containing the corresponding single-chain fragment antibody (scFv). The approach is illustrated with the small hapten fluorescein (FL) and a high-affinity anti-FL scFv fused to two different proteins in yeast and murine neuronal cell line N2a.