10.1021/am800043z.s001
Erin M. Fenton
Erin M.
Fenton
Monica R. Mascarenas
Monica R.
Mascarenas
Gabriel P. López
Gabriel P.
López
Scott S. Sibbett
Scott S.
Sibbett
Multiplex Lateral-Flow Test Strips Fabricated by Two-Dimensional Shaping
American Chemical Society
2009
bioassay reagents
strip
assay surfaces
evaporation
ii
dehydration
multiplex
plastic cassette holders
laminar composites
adoption
ShapingWe
operator error
iii
nitrocellulose media
contamination
device
manufacturing
candelabra
Fabricated
Multiplex
Strip
prototype development
fabrication method
dimension
polyester plastic films
2009-01-28 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Multiplex_Lateral_Flow_Test_Strips_Fabricated_by_Two_Dimensional_Shaping/2882620
We have fabricated paper- and nitrocellulose-based lateral-flow devices that are shaped in two dimensions by a computer-controlled knife. The resulting star, candelabra, and other structures are spotted with multiple bioassay reagents to produce multiplex lateral-flow assays. We have also fabricated laminar composites in which porous nitrocellulose media are sandwiched between vinyl and polyester plastic films. This minimizes evaporation, protects assay surfaces from contamination and dehydration, and eliminates the need for the conventional hard plastic cassette holders that are typically used to package commercial lateral-flow diagnostic strips. The reported fabrication method is novel, low-cost, and well-suited to (i) fabrication and adoption in resource-poor areas, (ii) prototype development, (iii) high-volume manufacturing, and (iii) improving rates of operator error.