High-Affinity Labeling and Tracking of Individual Histidine-Tagged Proteins in Live Cells Using Ni<sup>2+</sup> Tris-nitrilotriacetic Acid Quantum Dot Conjugates Victor Roullier Samuel Clarke Changjiang You Fabien Pinaud Géraldine Gouzer Dirk Schaible Valérie Marchi-Artzner Jacob Piehler Maxime Dahan 10.1021/nl9001298.s001 https://acs.figshare.com/articles/journal_contribution/High_Affinity_Labeling_and_Tracking_of_Individual_Histidine_Tagged_Proteins_in_Live_Cells_Using_Ni_sup_2_sup_Tris_nitrilotriacetic_Acid_Quantum_Dot_Conjugates/2872456 Investigation of many cellular processes using fluorescent quantum dots (QDs) is hindered by the nontrivial requirements for QD surface functionalization and targeting. To address these challenges, we designed, characterized and applied QD−trisNTA, which integrates tris-nitrilotriacetic acid, a small and high-affinity recognition unit for the ubiquitous polyhistidine protein tag. Using QD−trisNTA, we demonstrate two-color QD tracking of the type-1 interferon receptor subunits in live cells, potentially enabling direct visualization of protein−protein interactions at the single molecule level. 2009-03-11 00:00:00 Live Cells nontrivial requirements polyhistidine protein tag Quantum Dot ConjugatesInvestigation QD surface functionalization quantum dots interferon receptor subunits molecule level