10.1021/pr800937k.s003 Verena S. Meyer Verena S. Meyer Oliver Drews Oliver Drews Marc Günder Marc Günder Jörg Hennenlotter Jörg Hennenlotter Hans-Georg Rammensee Hans-Georg Rammensee Stefan Stevanovic Stefan Stevanovic Identification of Natural MHC Class II Presented Phosphopeptides and Tumor-Derived MHC Class I Phospholigands American Chemical Society 2009 phosphorylated MHC class cell carcinoma phosphorylated MHC class II ligands CXCR tumor tissue transmembrane receptors novel phosphorylation sites PhospholigandsMHC molecules T lymphocytes Natural MHC Class II Presented Phosphopeptides 2009-07-06 00:00:00 Dataset https://acs.figshare.com/articles/dataset/Identification_of_Natural_MHC_Class_II_Presented_Phosphopeptides_and_Tumor_Derived_MHC_Class_I_Phospholigands/2845684 MHC molecules present protein-derived peptides to T lymphocytes. By developing TiO<sub>2</sub>-based microcentrifugation columns, we identified the first phosphorylated MHC class I ligands from tumor tissue (renal cell carcinoma) and, by comparison to healthy renal tissue, found one Brf1-derived ligand as potentially tumor-associated. We further discovered the first natural phosphorylated MHC class II ligands. They revealed several novel phosphorylation sites of significant transmembrane receptors, such as Frizzled 6, CXCR4 and CD20.