%0 Journal Article %A Zhang, Leilei %A Yan, Yu %A Liu, Zijie %A Abliz, Zeper %A Liu, Gang %D 2009 %T Identification of Peptide Substrate and Small Molecule Inhibitors of Testis-Specific Serine/Threonine Kinase1 (TSSK1) By the Developed Assays %U https://acs.figshare.com/articles/journal_contribution/Identification_of_Peptide_Substrate_and_Small_Molecule_Inhibitors_of_Testis_Specific_Serine_Threonine_Kinase1_TSSK1_By_the_Developed_Assays/2841439 %R 10.1021/jm9002846.s001 %2 https://acs.figshare.com/ndownloader/files/4539172 %K TSSK 1 %K Pep 8 %K LC %K Peptide Substrate %K TSSK 1 inhibitors screening %K Small Molecule Inhibitors %K kinase assay %K peptide substrate %X In this paper, a peptide substrate (Pep8) of TSSK1 is identified. Using Pep8 as a substrate, two homogeneous and efficient assays for TSSK1 inhibitors screening have been developed, including luminescent kinase assay and LC−MS-based high-throughput assay. Two classes of compounds were identified that are able to efficiently inhibit phosphorylation catalyzed by TSSK1. %I ACS Publications