10.1021/jm9002846.s001 Leilei Zhang Leilei Zhang Yu Yan Yu Yan Zijie Liu Zijie Liu Zeper Abliz Zeper Abliz Gang Liu Gang Liu Identification of Peptide Substrate and Small Molecule Inhibitors of Testis-Specific Serine/Threonine Kinase1 (TSSK1) By the Developed Assays American Chemical Society 2009 TSSK 1 Pep 8 LC Peptide Substrate TSSK 1 inhibitors screening Small Molecule Inhibitors kinase assay peptide substrate 2009-07-23 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Identification_of_Peptide_Substrate_and_Small_Molecule_Inhibitors_of_Testis_Specific_Serine_Threonine_Kinase1_TSSK1_By_the_Developed_Assays/2841439 In this paper, a peptide substrate (Pep8) of TSSK1 is identified. Using Pep8 as a substrate, two homogeneous and efficient assays for TSSK1 inhibitors screening have been developed, including luminescent kinase assay and LC−MS-based high-throughput assay. Two classes of compounds were identified that are able to efficiently inhibit phosphorylation catalyzed by TSSK1.