10.1021/jm9002846.s001
Leilei Zhang
Leilei
Zhang
Yu Yan
Yu
Yan
Zijie Liu
Zijie
Liu
Zeper Abliz
Zeper
Abliz
Gang Liu
Gang
Liu
Identification of Peptide Substrate and Small Molecule Inhibitors of Testis-Specific Serine/Threonine Kinase1 (TSSK1) By the Developed Assays
American Chemical Society
2009
TSSK 1
Pep 8
LC
Peptide Substrate
TSSK 1 inhibitors screening
Small Molecule Inhibitors
kinase assay
peptide substrate
2009-07-23 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Identification_of_Peptide_Substrate_and_Small_Molecule_Inhibitors_of_Testis_Specific_Serine_Threonine_Kinase1_TSSK1_By_the_Developed_Assays/2841439
In this paper, a peptide substrate (Pep8) of TSSK1 is identified. Using Pep8 as a substrate, two homogeneous and efficient assays for TSSK1 inhibitors screening have been developed, including luminescent kinase assay and LC−MS-based high-throughput assay. Two classes of compounds were identified that are able to efficiently inhibit phosphorylation catalyzed by TSSK1.