10.1021/ic901407d.s001
Mohd Zulkefeli
Mohd
Zulkefeli
Tetsuya Sogon
Tetsuya
Sogon
Kei Takeda
Kei
Takeda
Eiichi Kimura
Eiichi
Kimura
Shin Aoki
Shin
Aoki
Design and Synthesis of a Stable Supramolecular Trigonal Prism Formed by the Self-Assembly of a Linear Tetrakis(Zn<sup>2+</sup>−cyclen) Complex and Trianionic Trithiocyanuric Acid in Aqueous Solution and Its Complexation with DNA (Cyclen = 1,4,7,10-Tetraazacyclododecane)
American Chemical Society
2009
polycationic template
UV titrations
Trianionic Trithiocyanuric
MS measurements
trianionic trithiocyanurate
Stable Supramolecular Trigonal Prism
Zn 4L
prism configuration
binding assays
solution
TCA
potentiometric pH
Aqueous Solution
ethidium bromide
building blocks
prism functions
gel mobility shift assays
DNA
1 H NMR titration
2009-10-05 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Design_and_Synthesis_of_a_Stable_Supramolecular_Trigonal_Prism_Formed_by_the_Self_Assembly_of_a_Linear_Tetrakis_Zn_sup_2_sup_cyclen_Complex_and_Trianionic_Trithiocyanuric_Acid_in_Aqueous_Solution_and_Its_Complexation_with_DNA_Cyclen_1_4_7_10_Tetraazacyclo/2823838
A new supramolecular complex, {(Zn<sub>4</sub>L<sup>4</sup>)<sub>3</sub>−(TCA<sup>3−</sup>)<sub>4</sub>}<sup>12+</sup>, was designed and synthesized by the 3:4 self-assembly of a linear tetrakis(Zn<sup>2+</sup>−cyclen) complex (Zn<sub>4</sub>L<sup>4</sup>)<sup>8+</sup> and trianionic trithiocyanurate (TCA<sup>3−</sup>) in aqueous solution (cyclen = 1,4,7,10-tetraazacyclododecane). The {(Zn<sub>4</sub>L<sup>4</sup>)<sub>3</sub>−(TCA<sup>3−</sup>)<sub>4</sub>}<sup>12+</sup> complex, which should have a trigonal prism configuration, was found to be very stable in aqueous solution at neutral pH and 25 °C, as evidenced by <sup>1</sup>H NMR titration, potentiometric pH and UV titrations, and MS measurements. The complex does not dissociate into the starting building blocks in the presence of Zn<sup>2+</sup>-binding anions such as phosphates and double-stranded DNA. The results of the competitive binding assays with ethidium bromide and calf-thymus DNA, thermal melting experiments, gel mobility shift assays, and dynamic light-scattering data strongly indicated that the trigonal prism functions as a polycationic template to induce the aggregation of double-stranded DNA.