%0 Generic
%A Xiao, Hui
%A Bissati, Kamal El
%A Verdier-Pinard, Pascal
%A Burd, Berta
%A Zhang, Hongshan
%A Kim, Kami
%A Fiser, Andras
%A Angeletti, Ruth Hogue
%A Weiss, Louis M.
%D 2010
%T Post-Translational Modifications to Toxoplasma gondii α- and β-Tubulins Include Novel C-Terminal Methylation
%U https://acs.figshare.com/articles/dataset/Post_Translational_Modifications_to_i_Toxoplasma_gondii_i_and_Tubulins_Include_Novel_C_Terminal_Methylation/2802355
%R 10.1021/pr900699a.s001
%2 https://acs.figshare.com/ndownloader/files/4496764
%K foreskin fibroblasts
%K detyrosinated tubulin
%K acid residue Tyr 453
%K invasion process
%K mass spectrometry
%K Methylated tubulins
%K acid residues
%K gondii tubulin PTMs
%K NIH Category B priority pathogen
%K host cells
%K subpellicular microtubules
%K Detyrosinated tubulin
%K genome encodes
%K apical region
%K basal end
%X Toxoplasma gondii is an apicomplexan of both medical and veterinary importance which is classified as an NIH Category B priority pathogen. It is best known for its ability to cause congenital infection in immune competent hosts and encephalitis in immune compromised hosts. The highly stable and specialized microtubule-based cytoskeleton participates in the invasion process. The genome encodes three isoforms of both α- and β-tubulin and we show that the tubulin is extensively altered by specific post-translational modifications (PTMs) in this paper. T. gondii tubulin PTMs were analyzed by mass spectrometry and immunolabeling using specific antibodies. The PTMs identified on α-tubulin included acetylation of Lys40, removal of the last C-terminal amino acid residue Tyr453 (detyrosinated tubulin) and truncation of the last five amino acid residues. Polyglutamylation was detected on both α- and β-tubulins. An antibody directed against mammalian α-tubulin lacking the last two C-terminal residues (Δ2-tubulin) labeled the apical region of this parasite. Detyrosinated tubulin was diffusely present in subpellicular microtubules and displayed an apparent accumulation at the basal end. Methylation, a PTM not previously described on tubulin, was also detected. Methylated tubulins were not detected in the host cells, human foreskin fibroblasts, suggesting that this may be a modification specific to the Apicomplexa.
%I ACS Publications