10.1021/pr100124b.s005 Filippos Michopoulos Filippos Michopoulos Georgios Theodoridis Georgios Theodoridis Christopher J. Smith Christopher J. Smith Ian D. Wilson Ian D. Wilson Metabolite Profiles from Dried Biofluid Spots for Metabonomic Studies using UPLC Combined with oaToF-MS American Chemical Society 2010 electrospray ionization sample UPLC Biofluid Spots paper substrate urine spots ESI Metabonomic Studies Metabolite Profiles plasma spots 2010-06-04 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Metabolite_Profiles_from_Dried_Biofluid_Spots_for_Metabonomic_Studies_using_UPLC_Combined_with_oaToF_MS/2764363 The potential of dried blood, plasma and urine spots, deposited on a paper substrate, combined with reversed-phase ultra performance liquid chromatography and orthogonal acceleration time-of-flight mass spectrometry (UPLC-oaToFMS), with electrospray ionization (ESI), has been investigated for global metabolic profiling. When compared to analysis using protein precipitated plasma, both blood and plasma spots gave comparable profiles and numbers of ions etc., using both positive and negative ESI. In the case of urine the results for spots obtained in positive ESI were also comparable to those of the untreated sample, but with negative ESI, a significant reduction in ions was noted for spotted samples. These preliminary data suggest that, with further optimization, biofluid spotting may provide an alternative to conventional methods for this type of work in suitable applications.