10.1021/pr100124b.s005
Filippos Michopoulos
Filippos
Michopoulos
Georgios Theodoridis
Georgios
Theodoridis
Christopher J. Smith
Christopher J.
Smith
Ian D. Wilson
Ian D.
Wilson
Metabolite Profiles from Dried Biofluid Spots for Metabonomic Studies using UPLC Combined with oaToF-MS
American Chemical Society
2010
electrospray ionization
sample
UPLC
Biofluid Spots
paper substrate
urine spots
ESI
Metabonomic Studies
Metabolite Profiles
plasma spots
2010-06-04 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Metabolite_Profiles_from_Dried_Biofluid_Spots_for_Metabonomic_Studies_using_UPLC_Combined_with_oaToF_MS/2764363
The potential of dried blood, plasma and urine spots, deposited on a paper substrate, combined with reversed-phase ultra performance liquid chromatography and orthogonal acceleration time-of-flight mass spectrometry (UPLC-oaToFMS), with electrospray ionization (ESI), has been investigated for global metabolic profiling. When compared to analysis using protein precipitated plasma, both blood and plasma spots gave comparable profiles and numbers of ions etc., using both positive and negative ESI. In the case of urine the results for spots obtained in positive ESI were also comparable to those of the untreated sample, but with negative ESI, a significant reduction in ions was noted for spotted samples. These preliminary data suggest that, with further optimization, biofluid spotting may provide an alternative to conventional methods for this type of work in suitable applications.