10.1021/la1008895.s001 Ana Sala Ana Sala Martin Ehrbar Martin Ehrbar Diana Trentin Diana Trentin Ronald G. Schoenmakers Ronald G. Schoenmakers Janos Vörös Janos Vörös Franz E. Weber Franz E. Weber Enzyme Mediated Site-Specific Surface Modification American Chemical Society 2010 growth factors surface RGD poly protein PEG peptide VEGF glycol biotin streptavidin interaction 2010-07-06 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Enzyme_Mediated_Site_Specific_Surface_Modification/2755483 Stable tethering of bioactive peptides like RGD to surfaces can be achieved via chemical bonding, biotin streptavidin interaction, or photocross-linking. More challenging is the immobilization of proteins, since methods applied to immobilize peptides are either not specific or versatile enough or might even compromise the protein’s bioactivity. To overcome this limitation, we have employed a scheme that by enzymatic (transglutaminase) reaction allows the site-directed and site-specific coupling of growth factors and other molecules to nonfouling poly(l-lysine)-graft-poly(ethylene glycol) (PLL-<i>g</i>-PEG) coated surfaces under physiological conditions. By our modular and flexible design principle, we are able to functionalize these surfaces directly with peptides and growth factors or precisely position poly(ethylene glycol) (PEG)-like hydrogels for the presentation of growth factors as exemplified with vascular endothelial growth factor (VEGF).