10.1021/la1008895.s001
Ana Sala
Ana
Sala
Martin Ehrbar
Martin
Ehrbar
Diana Trentin
Diana
Trentin
Ronald G. Schoenmakers
Ronald G.
Schoenmakers
Janos Vörös
Janos
Vörös
Franz E. Weber
Franz E.
Weber
Enzyme Mediated Site-Specific Surface Modification
American Chemical Society
2010
growth factors
surface
RGD
poly
protein
PEG
peptide
VEGF
glycol
biotin streptavidin interaction
2010-07-06 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Enzyme_Mediated_Site_Specific_Surface_Modification/2755483
Stable tethering of bioactive peptides like RGD to surfaces can be achieved via chemical bonding, biotin streptavidin interaction, or photocross-linking. More challenging is the immobilization of proteins, since methods applied to immobilize peptides are either not specific or versatile enough or might even compromise the protein’s bioactivity. To overcome this limitation, we have employed a scheme that by enzymatic (transglutaminase) reaction allows the site-directed and site-specific coupling of growth factors and other molecules to nonfouling poly(l-lysine)-graft-poly(ethylene glycol) (PLL-<i>g</i>-PEG) coated surfaces under physiological conditions. By our modular and flexible design principle, we are able to functionalize these surfaces directly with peptides and growth factors or precisely position poly(ethylene glycol) (PEG)-like hydrogels for the presentation of growth factors as exemplified with vascular endothelial growth factor (VEGF).