Nuclear Magnetic Resonance Structure of the Prohead RNA E-Loop Hairpin,
Steven Harris
Susan J. Schroeder
10.1021/bi100393r.s001
https://acs.figshare.com/articles/journal_contribution/Nuclear_Magnetic_Resonance_Structure_of_the_Prohead_RNA_E_Loop_Hairpin_/2748598
The <i>Bacillus subtilis</i> phage ϕ29 packaging motor requires prohead RNA for genome encapsidation. The nuclear magnetic resonance structure of the prohead RNA E-loop hairpin, r(5′AUUGAGUU), is presented and compared to predictions from MC-SYM. The prohead RNA E-loop hairpins contain sequences similar to rRNA hairpins. Comparison of predicted and experimentally determined prohead and ribosomal hairpin structures reveals that sequence similarity is a stronger determinant of hairpin structural similarity than grouping similar types of RNA. All the hairpins contain a U-turn motif but differ in the first noncanonical pair and backbone orientation. These structures provide benchmarks for further improvements in RNA structure predictions from sequence.
2010-07-27 00:00:00
RNA structure predictions
backbone orientation
resonance structure
genome encapsidation
noncanonical pair
hairpin structures
Resonance Structure
sequence similarity
rRNA hairpins
prohead RNA