10.1021/ja106506k.s003
Gufeng Wang
Gufeng
Wang
Wei Sun
Wei
Sun
Yong Luo
Yong
Luo
Ning Fang
Ning
Fang
Resolving Rotational Motions of Nano-objects in Engineered Environments and Live Cells with Gold Nanorods and Differential Interference Contrast Microscopy
American Chemical Society
2010
Resolving Rotational Motions
3 D space
DIC microscopy
cytoskeleton
orientation probes
Engineered Environments
combination
Gold Nanorods
anisotropic
motor proteins
surface
Live Cells
gold nanorod probes
interference contrast
Differential Interference Contrast MicroscopyGold nanorods
video rate
2010-11-24 00:00:00
Media
https://acs.figshare.com/articles/media/Resolving_Rotational_Motions_of_Nano_objects_in_Engineered_Environments_and_Live_Cells_with_Gold_Nanorods_and_Differential_Interference_Contrast_Microscopy/2710234
Gold nanorods are excellent orientation probes due to their anisotropic optical properties. Their dynamic rotational motion in the 3D space can be disclosed with Nomarski-type differential interference contrast (DIC) microscopy. We demonstrate that by using the combination of gold nanorod probes and DIC microscopy, we are able to resolve rotational motions of nano-cargos transported by motor proteins at video rate not only on engineered surfaces but also on cytoskeleton tracks in live cells.