%0 Journal Article %A Anagnostopoulos, Athanasios K. %A Dimas, Konstantinos S. %A Papathanassiou, Chrissa %A Braoudaki, Maria %A Anastasiadou, Ema %A Vougas, Konstantinos %A Karamolegou, Kalliopi %A Kontos, Harry %A Prodromou, Neofytos %A Tzortzatou-Stathopoulou, Fotini %A Tsangaris, George Th. %D 2011 %T Proteomics Studies of Childhood Pilocytic Astrocytoma %U https://acs.figshare.com/articles/journal_contribution/Proteomics_Studies_of_Childhood_Pilocytic_Astrocytoma/2656495 %R 10.1021/pr200024m.s004 %2 https://acs.figshare.com/ndownloader/files/4314160 %K protein profiles %K brain tumors %K childhood pilocytic astrocytoma occurrence %K Proteomics Studies %K Proteomics analysis %K Childhood Pilocytic AstrocytomaChildhood pilocytic astrocytoma %K brain tumor %K brain tissues %K ERK %K evaluation %K bioinformatics approaches %K proteomic data %K cancer research %K tissue samples %K proteome content %K protein expression %K MAPK pathway %K genomic hybridization arrays %K bioinformatics database construction %K novel findings %X Childhood pilocytic astrocytoma is the most frequent brain tumor affecting children. Proteomics analysis is currently considered a powerful tool for global evaluation of protein expression and has been widely applied in the field of cancer research. In the present study, a series of proteomics, genomics, and bioinformatics approaches were employed to identify, classify and characterize the proteome content of low-grade brain tumors as it appears in early childhood. Through bioinformatics database construction, protein profiles generated from pathological tissue samples were compared against profiles of normal brain tissues. Additionally, experiments of comparative genomic hybridization arrays were employed to monitor for genetic aberrations and sustain the interpretation and evaluation of the proteomic data. The current study confirms the dominance of MAPK pathway for the childhood pilocytic astrocytoma occurrence and novel findings regarding the ERK-2 expression are reported. %I ACS Publications