10.1021/la201240h.s001 A. Chatzipavlidis A. Chatzipavlidis P. Bilalis P. Bilalis E. K. Efthimiadou E. K. Efthimiadou N. Boukos N. Boukos G. C. Kordas G. C. Kordas Sacrificial Template-Directed Fabrication of Superparamagnetic Polymer Microcontainers for pH-Activated Controlled Release of Daunorubicin American Chemical Society 2011 step concerns electron Superparamagnetic Polymer Microcontainers microscopy sample magnetometry PMAA microspheres acid XRD DLS release rate formation PMAA layer nanoparticles entrapped TEM SEM release system coprecipitation method microcontainer step deals pH values distillation precipitation polymerization VSM 2011-07-05 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Sacrificial_Template_Directed_Fabrication_of_Superparamagnetic_Polymer_Microcontainers_for_pH_Activated_Controlled_Release_of_Daunorubicin/2633605 Magnetic pH-sensitive microcontainers were produced by a four-step process. The first step involves the synthesis of citrate-modified magnetic nanoparticles via the coprecipitation method. The second step consists of the encapsulation of magnetic nanoparticles in non-cross-linked poly(methacrylic acid) (PMAA) microspheres through distillation precipitation polymerization, resulting in a core/shell structure. The third step concerns the formation of a poly(<i>N</i>,<i>N</i>′-methylenebis(acrylamide)-<i>co</i>-mathacrylic acid) (P(MBAAm-<i>co</i>-MAA)) layer on the surface of magnetic PMAA microspheres by second distillation precipitation polymerization in order to produce a trilayer hybrid microsphere. The last step deals with the removal of PMAA layer in ethanol and formation of a stable P(MBAAm<i>-<i>co</i>-</i>MAA) microcontainer with magnetic nanoparticles entrapped inside the formed cavity. This process is simple and leads to the formation of superparamagnetic pH-sensitive microcontainers. The structure and properties of the magnetic microcontainers were investigated by X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), vibrating sample magnetometry (VSM), and dynamic light scattering (DLS) to determine the functionalities of the hybrid structure. The magnetic pH-sensitive microcontainers were loaded with Daunorubicin and tested with respect to release rate at different pH values in order to evaluate their functionality as controlled release system.