10.1021/np200056e.s001
Tzong-Huei Lee
Tzong-Huei
Lee
Chung-Kuang Lu
Chung-Kuang
Lu
Guei-Jane Wang
Guei-Jane
Wang
Ya-Chih Chang
Ya-Chih
Chang
Wen-Bin Yang
Wen-Bin
Yang
Yu-Min Ju
Yu-Min
Ju
Sesquiterpene Glycosides from <i>Cosmospora joca</i>
American Chemical Society
2011
100 μ M
nitric oxide
sesquiterpene moiety
Sesquiterpene Glycosides
Cosmospora joca
RAW
spectroscopic data analyses
monosaccharide composition analyses
LPS
ethyl acetate
Cosmospora jocaFive novel sesquiterpene glycosides
monosaccharide chirality analyses
configuration
NOESY assignments
concentration value
2011-07-22 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Sesquiterpene_Glycosides_from_i_Cosmospora_joca_i_/2629074
Five novel sesquiterpene glycosides, namely, cosmosporasides A–E (<b>1</b>–<b>5</b>), were isolated from the ethyl acetate extract of the fermented broth of <i>Cosmospora joca</i>. The structures of <b>1</b>–<b>5</b> were elucidated on the basis of spectroscopic data analyses, monosaccharide composition analyses, and monosaccharide chirality analyses. The relative configuration of the sesquiterpene moiety of <b>3</b>–<b>5</b> was determined by <i>J</i>-based configuration analyses and supported by NOESY assignments. The inhibitory effects of <b>1</b>–<b>5</b> on the nitric oxide (NO) production in lipopolysaccharide (LPS)-activated murine macrophage RAW264.7 cells were evaluated; all except <b>3</b> inhibited NO production at 100 μM. <b>4</b> was the most potent, with an average maximum inhibition and a median inhibitory concentration value of 71.70 ± 1.23% and 65.98 ± 0.53 μM, respectively.