Vallée-Bélisle, Alexis Bonham, Andrew J. Reich, Norbert O. Ricci, Francesco Plaxco, Kevin W. Transcription Factor Beacons for the Quantitative Detection of DNA Binding Activity The development of convenient, real-time probes for monitoring protein function in biological samples represents an important challenge of the postgenomic era. In response, we introduce here “transcription factor beacons,” binding-activated fluorescent DNA probes that signal the presence of specific DNA-binding activities. As a proof of principle, we present beacons for the rapid, sensitive detection of three transcription factors (TATA Binding Protein, Myc-Max, and NF-κB), and measure binding activity directly in crude nuclear extracts. DNA Binding ActivityThe development;presence;sample;monitoring protein function;postgenomic era;extract;TATA Binding Protein;DNA probes;NF;measure binding activity;transcription factors;principle;Quantitative Detection;crude;beacon;response;Transcription Factor Beacons;detection;challenge;signal;proof 2011-09-07
    https://acs.figshare.com/articles/journal_contribution/Transcription_Factor_Beacons_for_the_Quantitative_Detection_of_DNA_Binding_Activity/2616634
10.1021/ja204775k.s001