10.1021/bc2003378.s001
Shoichiro Asayama
Shoichiro
Asayama
Satoshi Nishinohara
Satoshi
Nishinohara
Hiroyoshi Kawakami
Hiroyoshi
Kawakami
Zinc-Chelated Poly(1-vinylimidazole) and a Carbohydrate Ligand Polycation Form DNA Ternary Complexes for Gene Delivery
American Chemical Society
2011
recognition
cell surface receptors
gene expression
Carbohydrate Ligand Polycation Form DNA Ternary Complexes
carbohydrate ligand polycation
DNA ternary complexes
dissociation
2011-09-21 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Zinc_Chelated_Poly_1_vinylimidazole_and_a_Carbohydrate_Ligand_Polycation_Form_DNA_Ternary_Complexes_for_Gene_Delivery/2612017
Zinc-chelated poly(1-vinylimidazole) (PVIm-Zn) and a carbohydrate ligand polycation, a poly(l-lysine) conjugated with lactose molecules (PLL-Lac), have formed DNA ternary complexes for gene delivery. The particle size of the PVIm-Zn/DNA complexes with negative zeta potential was decreased by the addition of the PLL-Lac. The resulting PLL-Lac/PVIm-Zn/DNA ternary complexes, which exhibited the pH-dependent dissociation of the PLL-Lac, mediated more gene expression than the PVIm/DNA binary complexes. The PLL-Lac/PVIm-Zn/DNA complexes with the specific recognition of cell surface receptors mediated the highest gene expression without cytotoxicity at a relatively lower charge ratio (positive/negative = 2.5). These results suggest that the pH-dependent dissociation of the carbohydrate ligands after the recognition of cell surface receptors, including the physicochemical and biochemical function of PVIm-Zn, played an important role in gene expression.