SILAC Compatible Strain of <i>Pichia pastoris</i> for Expression of Isotopically Labeled Protein Standards and Quantitative Proteomics Ryan J. Austin Rolf E. Kuestner Debbie K. Chang Knut R. Madden Daniel B. Martin 10.1021/pr200551e.s001 https://acs.figshare.com/articles/journal_contribution/SILAC_Compatible_Strain_of_i_Pichia_pastoris_i_for_Expression_of_Isotopically_Labeled_Protein_Standards_and_Quantitative_Proteomics/2590606 The methylotrophic yeast <i>Pichia pastoris</i> is a powerful eukaryotic platform for the production of heterologous protein. Recent publication of the <i>P. pastoris</i> genome has facilitated strain development toward biopharmaceutical and environmental science applications and has advanced the organism as a model system for the study of peroxisome biogenesis and methanol metabolism. Here we report the development of a <i>P. pastoris</i> arg-/lys<i>-</i> auxotrophic strain compatible with SILAC (stable isotope labeling by amino acids in cell culture) proteomic studies, which is capable of generating large quantities of isotopically labeled protein for mass spectrometry-based biomarker measurements. We demonstrate the utility of this strain to produce high purity human serum albumin uniformly labeled with isotopically heavy arginine and lysine. In addition, we demonstrate the first quantitative proteomic analysis of methanol metabolism in <i>P. pastoris</i>, reporting new evidence for a malate–aspartate NADH shuttle mechanism in the organism. This strain will be a useful model organism for the study of metabolism and peroxisome generation. 2011-11-04 00:00:00 Quantitative ProteomicsThe methylotrophic yeast Pichia pastoris SILAC Compatible Strain methanol metabolism NADH strain protein isotopically peroxisome Isotopically Labeled Protein Standards proteomic model organism