Analysis of Transcriptional Factors and Regulation Networks in Laryngeal Squamous Cell Carcinoma Patients with Lymph Node Metastasis
Geng-Ming Cai
Dong-Hai Huang
Yao-Zhang Dai
Yong Liu
Lei-Ming Pi
Hao-Lei Tan
Li-Jun Liu
Yun-Xia Lv
Chang-Yun Yu
Ping-Qing Tan
Yong-Quan Tian
Georgia Z. Chen
Xin Zhang
10.1021/pr200831g.s004
https://acs.figshare.com/articles/dataset/Analysis_of_Transcriptional_Factors_and_Regulation_Networks_in_Laryngeal_Squamous_Cell_Carcinoma_Patients_with_Lymph_Node_Metastasis/2554261
The present study was to identify and quantitate differentially expressed proteins in laryngeal squamous cell carcinoma (LSCC) tissues with or without lymph node metastasis and to explore transcriptional factors and regulation networks associated with the process. Tissue specimens were taken from 20 patients with LSCC, including 10 cases of LSCC without metastasis LSCC (N0) and 10 cases of LSCC with metastasis LSCC (Nx). Among the 643 unique proteins identified by using iTRAQ labeling and quantitative proteomic technology, 389 proteins showed an abundance change in LSCC (Nx) as compared to LSCC (N0). Cytoskeleton remodeling, cell adhesion, and immune response activation were found to be the main processes in LSCC metastasis. The construction of transcription regulation networks identified key transcription regulators for lymph node metastasis of LSCC, including Sp1, c-myc, and p53, which may affect LSCC metastasis through the epithelial–mesenchymal transition. Furthermore, our results suggest that ubiquitination may be a critical factor in the networks. The present study provides insights into transcriptional factors and regulation networks involved in LSCC metastasis, which may lead to new strategies for treatment of LSCC metastasis.
2016-02-22 07:10:33
Lymph Node MetastasisThe
transcription regulation networks
regulation networks
Laryngeal Squamous Cell Carcinoma Patients
10 cases
lymph node metastasis
transcriptional factors
LSCC metastasis
metastasis LSCC