10.1021/ja301332t.s004
Kyle Marchuk
Kyle
Marchuk
Yijun Guo
Yijun
Guo
Wei Sun
Wei
Sun
Javier Vela
Javier
Vela
Ning Fang
Ning
Fang
High-Precision Tracking
with Non-blinking Quantum
Dots Resolves Nanoscale Vertical Displacement
American Chemical Society
2012
incident angles
reflection fluorescence microscope
microtubule
decay functions
emission intensity profile
fluorescence intensity decay curves
assay conditions
NBQD
2012-04-11 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/High_Precision_Tracking_with_Non_blinking_Quantum_Dots_Resolves_Nanoscale_Vertical_Displacement/2531923
Novel non-blinking quantum dots (NBQDs) were utilized
in three-dimensional
super-localization, high-precision tracking applications under an
automated scanning-angle total internal reflection fluorescence microscope
(SA-TIRFM). NBQDs were randomly attached to stationary microtubules
along the radial axis under gliding assay conditions. By automatically
scanning through a wide range of incident angles with different evanescent-field
layer thicknesses, the fluorescence intensity decay curves were obtained.
Fit with theoretical decay functions, the absolute vertical positions
were determined with sub-10-nm localization precision. The emission
intensity profile of the NBQDs attached to kinesin-propelled microtubules
was used to resolve the self-rotation of gliding microtubules within
a small vertical distance of ∼50 nm. We demonstrate the applicability
of NBQDs in high-precision fluorescence imaging experiments.