10.1021/es300550h.s001
Guillaume Jubeaux
Guillaume
Jubeaux
Fabien Audouard-Combe
Fabien
Audouard-Combe
Romain Simon
Romain
Simon
Renaud Tutundjian
Renaud
Tutundjian
Arnaud Salvador
Arnaud
Salvador
Olivier Geffard
Olivier
Geffard
Arnaud Chaumot
Arnaud
Chaumot
Vitellogenin-like Proteins
among Invertebrate Species
Diversity: Potential of Proteomic Mass Spectrometry for Biomarker
Development
American Chemical Society
2012
taxa
ecotoxicology
assay
invertebrates
protein
mass spectrometry
biodiversity
Invertebrate Species Diversity
tissue
omics tools
peptide
biomarker development
species
Proteomic Mass Spectrometry
2012-06-05 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Vitellogenin_like_Proteins_among_Invertebrate_Species_Diversity_Potential_of_Proteomic_Mass_Spectrometry_for_Biomarker_Development/2517040
Cost-effective methodologies along with cross-species
applicability
constitute key points for biomarker development in ecotoxicology.
With the advent of cheaper affordable genomic techniques and high
throughput sequencing, omics tools could facilitate the assessment
of effects of environmental contaminants for all taxa biodiversity.
We assessed the potential of absolute quantification of proteins using
mass spectrometry to develop vitellogenin(Vg)-like protein assays
for invertebrates. We used available sequences in public databases
to rapidly identify Vg-proteotypic peptides in seven species from
different main taxa of protostome invertebrates (mollusk bivalves,
crustacean amphipods, branchiopods, copepods and isopods, and insect
diptera). Functional validation was performed by comparing proteomic
signals from reproductive female tissue samples and negative controls
(male or juvenile tissues). In a second part, we demonstrate in gammarids,
daphnids, drosophilids, and gastropods that the assay validated in
Vg-sequenced species can be applied to Vg-unsequenced species thanks
to the evolutionary conservation of Vg-proteotypic peptide motifs.
Finally, we discuss the relevance of mass spectrometry for biomarker
development (specific measurement, rapid development, transferability
across species). Our study supplies an illustration of the promising
strategy to address the challenge of biodiversity in ecotoxicology,
which consists in employing omics tools from comparative and evolutionary
perspectives.