A Facile Method to Synthesize
Histones with Posttranslational
Modification Mimics
Zhiyong
U. Wang
Yane-Shih Wang
Pei-Jing Pai
William K. Russell
David H. Russell
Wenshe R. Liu
10.1021/bi300535a.s001
https://acs.figshare.com/articles/journal_contribution/A_Facile_Method_to_Synthesize_Histones_with_Posttranslational_Modification_Mimics/2508757
Using an evolved pyrrolysyl-tRNA synthetase-tRNA<sup>Pyl</sup> pair,
a <i>Se</i>-alkylselenocysteine was genetically incorporated
into histone H3 with a high protein expression yield. Quantitative
oxidative elimination of <i>Se</i>-alkylselenocysteine followed
by Michael addition reactions with various thiol nucleophiles generated
biologically active mimics of H3 with posttranslational modifications
including lysine methylation, lysine acetylation, and serine phosphorylation.
2012-07-03 00:00:00
lysine methylation
Michael addition reactions
mimic
posttranslational modifications
serine phosphorylation
Quantitative oxidative elimination
Facile Method
Synthesize Histones
protein expression
Posttranslational Modification MimicsUsing
thiol nucleophiles
histone H 3
H 3
lysine acetylation