A Facile Method to Synthesize Histones with Posttranslational Modification Mimics Zhiyong U. Wang Yane-Shih Wang Pei-Jing Pai William K. Russell David H. Russell Wenshe R. Liu 10.1021/bi300535a.s001 https://acs.figshare.com/articles/journal_contribution/A_Facile_Method_to_Synthesize_Histones_with_Posttranslational_Modification_Mimics/2508757 Using an evolved pyrrolysyl-tRNA synthetase-tRNA<sup>Pyl</sup> pair, a <i>Se</i>-alkylselenocysteine was genetically incorporated into histone H3 with a high protein expression yield. Quantitative oxidative elimination of <i>Se</i>-alkylselenocysteine followed by Michael addition reactions with various thiol nucleophiles generated biologically active mimics of H3 with posttranslational modifications including lysine methylation, lysine acetylation, and serine phosphorylation. 2012-07-03 00:00:00 lysine methylation Michael addition reactions mimic posttranslational modifications serine phosphorylation Quantitative oxidative elimination Facile Method Synthesize Histones protein expression Posttranslational Modification MimicsUsing thiol nucleophiles histone H 3 H 3 lysine acetylation