10.1021/jp302734e.s001
Soma Banerjee
Soma
Banerjee
Masanori Tachiya
Masanori
Tachiya
Samir Kumar Pal
Samir Kumar
Pal
Caffeine-Mediated Detachment
of Mutagenic Ethidium
from Various Nanoscopic Micelles: An Ultrafast Förster Resonance
Energy Transfer Study
American Chemical Society
2012
epithelial cells
sodium dodecyl sulfate
charge
alteration
groove binder dye Hoeschst 33258
CTAB
Various Nanoscopic Micelles
biomimicking micelles
acceptor
cationic hexadecyltrimethylammonium bromide
fluorescence micrographs
presence
micelle changes
Fo
DNA
caffeine molecule
TX
donor
Mutagenic Ethidium
SDS
FRET efficiency
model
2012-07-12 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Caffeine_Mediated_Detachment_of_Mutagenic_Ethidium_from_Various_Nanoscopic_Micelles_An_Ultrafast_Fo_rster_Resonance_Energy_Transfer_Study/2505508
In the present study we explore the efficacy of caffeine
in dissociating
the ethidium (Et) molecule, a model DNA-intercalator as well as a
potential mutagen, from nanometer sized micelles of various charges.
Steady-state and picosecond-resolved spectroscopic studies on the
detachment of Et from various biomimicking micelles of different charges
(cationic hexadecyltrimethylammonium bromide (CTAB), neutral (polar)
Triton X-100 (TX-100), and anionic sodium dodecyl sulfate (SDS)) reveal
the specificity of the caffeine molecule for carrying out such dissociation.
The picosecond-resolved Förster resonance energy transfer (FRET)
studies between a DNA minor groove binder dye Hoeschst 33258 (H258,
donor) and Et (acceptor) have been employed to investigate the alteration
in their association in the presence of caffeine at the molecular
level. Analysis of our experimental results employing both the generalized
and the extended version of the well-known “Infelta–Tachiya
model” vividly illustrates how the distribution of Et along
with the equilibrium constant of its solubilization in the micelle
changes in the presence of caffeine in aqueous solution. Finally,
our fluorescence micrographs of squamous epithelial cells validate
the alteration of FRET efficiency between the donor and the acceptor
due to the release of the latter in the presence of caffeine.