10.1021/ac301523n.s001 Lin-Ai Tai Lin-Ai Tai Yu-Ting Kang Yu-Ting Kang Yu-Ching Chen Yu-Ching Chen Yu-Chao Wang Yu-Chao Wang Yu-Jing Wang Yu-Jing Wang Yu-Ting Wu Yu-Ting Wu Kuo-Liang Liu Kuo-Liang Liu Chiu-Yen Wang Chiu-Yen Wang Yu-Feng Ko Yu-Feng Ko Ching-Ya Chen Ching-Ya Chen Nai-Chun Huang Nai-Chun Huang Jen-Kun Chen Jen-Kun Chen Yong-Fen Hsieh Yong-Fen Hsieh Tri-Rung Yew Tri-Rung Yew Chung-Shi Yang Chung-Shi Yang Quantitative Characterization of Nanoparticles in Blood by Transmission Electron Microscopy with a Window-Type Microchip Nanopipet American Chemical Society 2012 Quantitative Characterization solution nanoparticle chamber width TEM holders sampling device Transmission Electron Microscopy microchip nanopipet particle concentration copper grids aggregation 2012-08-07 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Quantitative_Characterization_of_Nanoparticles_in_Blood_by_Transmission_Electron_Microscopy_with_a_Window_Type_Microchip_Nanopipet/2498503 Transmission electron microscopy (TEM) is a unique and powerful tool for observation of nanoparticles. However, due to the uneven spatial distribution of particles conventionally dried on copper grids, TEM is rarely employed to evaluate the spatial distribution of nanoparticles in aqueous solutions. Here, we present a microchip nanopipet with a narrow chamber width for sorting nanoparticles from blood and preventing the aggregation of the particles during the drying process, enabling quantitative analysis of their aggregation/agglomeration states and the particle concentration in aqueous solutions. This microchip is adaptable to all commercial TEM holders. Such a nanopipet proves to be a simple and convenient sampling device for TEM image-based quantitative characterization.